Isolation and characterisation of eight microsatellite markers of the thorn-tailed rayadito Aphrastura spinicaudata


Authors: Diego I. YÁÑEZ, Verónica QUIRICI, Gabriel J. CASTAÑO-VILLA, Elie POULIN and Rodrigo A. VÁSQUEZ


Published: Volume 62.1, June 2015. Pages 179-183.

Language: English

Keywords: Chile, Furnariidae, habitat specialist and population genetics


Eight novel microsatellites were isolated and characterised in the thorn-tailed rayadito Aphrastura spinicauda in order to evaluate genetic diversity in three contrasting populations, two of them at the boundaries of the geographic range of the species. All loci were found to be polymorphic and 3-15 alleles were found per locus. Expected and observed heterozygosity ranged from 0.26 to 0.89 and from 0.27 to 0.87, respectively. Global and pairwise Weir and Cockerham's Fst showed that genetic differentiation was greater between the northern population and the ones in central and southern Chile. The eight markers developed will be useful to study the genetic diversity of the thorn-tailed rayaditoacross its distribution.

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